immunohistochemistry goat polyclonal anti sclerostin  (R&D Systems)

Journal: Clinical Medicine Insights. Oncology

Article Title: Th9 and Th17 Cells in Human Ulcerative Colitis-Associated Dysplastic Lesions

doi: 10.1177/11795549241301358

Figure Lengend Snippet: Immunohistochemical (IHC) photographic presentation of CD3-positive T lymphocytes, PU.1-positive Th9, and IL-17A-positive Th17 cells in human UC and colitis-associated dysplastic tissues. Microscopic photos from the control sections showed that CD3-positive T lymphocytes were primarily observed in the lamina propria (black arrow-pointed cells in A), and intraepithelial CD3-positive T lymphocytes were also fund (black arrowhead-pointed cell in inserted enlarged image in A). As expected, increased CD3-positive T lymphocytes in the lamina propria (black arrow-pointed cells) and epithelium (black arrowhead-pointed cells in inserted enlarged images) were shown in both the UC (B) and colitis-associated dysplastic (C for ALD and D for NALD) sections. Microscopic photos of PU.1 IHC sections showed that increased PU.1-positive Th9 cells were observed in the lamina propria (black arrow-pointed cells) and epithelium (black arrowhead-pointed cells in inserted enlarged images) in both UC (F) and colitis-associated dysplastic (G for ALD and H for ALD) sections relative to the control section (E). Similarly, microscopic photos of IL-17A IHC sections showed that IL-17A-positive Th17 cells were increased in both the lamina propria (black arrow-pointed cells) and epithelium (black arrowhead-pointed cells in inserted enlarged images) in the UC (J) and colitis-associated dysplastic (K for ALD and L for NALD) sections as compared with the control section (I) (A to L IHC images, counterstained with hematoxylin, original magnification ×200). ALD indicates adenoma-like dysplasia; NALD, non-adenoma-like dysplasia; UC, ulcerative colitis.

Article Snippet: 1 polyclonal antibody (purchased from LifeSpan BioSciences Inc, Seattle, WA, USA) to label Th9 cells and goat anti-human IL-17A polyclonal antibody (Catalog No: AF-317-NA, clone Ile20Ala155, purchased from R&D System; Minneapolis, MN, USA) to label Th17 cells.

Techniques: Immunohistochemical staining, Control

Journal: Clinical Medicine Insights. Oncology

Article Title: Th9 and Th17 Cells in Human Ulcerative Colitis-Associated Dysplastic Lesions

doi: 10.1177/11795549241301358

Figure Lengend Snippet: Graph analysis of density grading scores of CD3-positive T lymphocytes, PU.1-positive Th9, and IL-17A-positive Th17 subset cells in human colitis-associated dysplastic tissues. Graphic statistical analysis of CD3-positive T lymphocytes, PU.1-positive Th9, and IL-17A-positive Th17 cells revealed that densities of CD3-positive T lymphocytes (A for positive cells in the lamina propria, B for positive cells in the epithelium), PU.1-positive Th9 cells (C for positive cells in the lamina propria, D for positive cells in the epithelium) and IL-17A-positive Th17 cells (E for positive cells in the lamina propria) were significantly increased in both the UC (gray bar) and UC-associated ALD (grid bar) and NALD (black bar) as compared with control (white bar, all P < .01 from the Kruskal-Wallis test). Interestingly, densities of PU.1-positive Th9 cells in both the lamina propria and epithelium of the NALD group were greatly higher than the UC group (refer to C and D, both P < .05 from the Mann-Whitney test) and the density of IL-17A-positive Th17 cells in the lamina propria of the NALD group was higher than that the UC group ( P < .05 from the Mann-Whitney test). However, densities of these cells were non-statistically changed ( P > .05), except the density of intraepithelial CD3-positive T lymphocytes (B, P < .05 from the Mann-Whitney test), between the ALD (grid bar) and NALD (black bar) in colitis-associated dysplastic sections (refer to F). ALD indicates adenoma-like dysplasia; NALD, non-adenoma-like dysplasia; UC, ulcerative colitis.

Article Snippet: 1 polyclonal antibody (purchased from LifeSpan BioSciences Inc, Seattle, WA, USA) to label Th9 cells and goat anti-human IL-17A polyclonal antibody (Catalog No: AF-317-NA, clone Ile20Ala155, purchased from R&D System; Minneapolis, MN, USA) to label Th17 cells.

Techniques: Control, MANN-WHITNEY